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KMID : 0380020150300060275
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Korean Journal of Biotechnology and Bioengineering
2015 Volume.30 No. 6 p.275 ~ p.282
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Antioxidant Activity of the Halophyte Ligustrum japonicum
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Baek Seung-Oh
Kim Ho-Jun
Jeong Hee-Jeong
Ju Eun-Shin
Kong Chang-Suk
Seo Young-Wan
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Abstract
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Dried samples of Ligustrum japonicum were extracted twice: with methylene chloride and with methanol (MeOH), respectively. The combined crude extracts were successively fractionated into n-hexane, 85% aqueous methanol (85% aq.MeOH), n-butanol (n-BuOH), and water fractions by liquid-liquid partition. Antioxidant activities of crude extract and its solvent fractions were evaluated by measuring authentic ONOO-, and ONOO- generated from 3-morpholinsydnonimine (SIN-1) as well as degree of occurrence of intracellular ROS in HT 1080 cells, and genomic DNA oxidation. The 85% aq.MeOH and n-BuOH fractions exhibited the good antioxidant activity. Further purification of the 85% aq.MeOH fracition led to the isolation of Oleanolic acid (1), Maslinic acid (2), and Ursolic acid (3). All compounds showed the significant antioxidant effects in all assay systems.
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KEYWORD
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Ligustrum japonicum, Antioxidant, Reactive oxygen species (ROS), Peroxynitrite
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